Protheine denaturation

Conformation (sebopeho) tsa ketane peptide e laela le e ikhethang oa ho e mong le e protheine. Ka maemo a khethehileng ho na le lekhalo le palo e kgolo ya litlamo tse tsitsisa mohaho tlhaloganya sebaka sa molek'hule komponeng. Ka lebaka leo, lekhalo le feletseng molek'hule kakaretso (kapa karolo e itseng mohlomong lona) nka sebōpeho sa kela tšohanyetso. Tshebetso ena o bitsoa "denaturation". Phetoho ena e ka mo lakalisa mocheso ho tloha ho likhato tse mashome a tšeletseng ho mashome a robeli. Kahoo, e mong le e molek'hule, lekhalo sephetho le ka fapana ho conformation ho tswa ho tse ling.

Denaturation liprotheine etsahala tlas'a tšusumetso ea mahlahana a leha e le efe nang le bokhoni ba ho timetsa litlamo bao e seng covalent. Tshebetso ena e ka etsahala tlas'a maemo a alkaline kapa asiti, ka holim 'a karolo mohato, tlas'a bohato la bang metsoako manyolo (phenols, alcohols le ba bang). protheine denaturation ka etsahala tlas'a tšusumetso ea guanidinium chloride kapa urea. Itse mahlahana a theha litlamo fokolang (hydrophobic, ionic, hydrogen) le lihlopha carbonyl kapa amino tsa peptide mokokotlo le palo ea lihlopha tsa litlhaka kahare amino acid e, a emeng ipatlele fumaneha protheine bona litlamo hydrogen hara limolek'hule le. Ka lebaka leo, phetoho e le hlaha ka sebopeho se mahareng le e phahameng.

Ho hanyetsa denaturing mahlahana a itšetlehile haholo-holo ka ho ba teng ka molek'hule ea metsoako le protheine la litlamo tsa disulfide. Trypsin sesitisa na litlamo tse tharo S - S. Tlas'a boemo ba hlaphoheloa ba protheine denaturation le hlaha kantle litšusumetso tse ling. Haeba o ile a beha mabapi maemong a moo ho kena moea o etsoang tsoa Sh-lihlopha tsa cysteine le sebopeho sa litlamo disulfide, ho conformation pele e tsosolositsoeng. Ho ba teng ha esita le e le 'ngoe disulfide tlamo e haholo ho eketsa botsitso ba ya sebopeho tlhaloganya sebaka.

protheine denaturation, ka tloaelo ho tsamaea le ea fokotseha ka solubility. Hammoho ena precipitate hangata e liforomo. E hlaha ka mokgwa wa "protheine curdled." Ka concentrations phahameng tsa metsoako ka tharollo, "coagulation" undergoes tharollo kaofela, e le, ka mohlala, sena se etsahala ha a phehile mahe. Ha denaturation tsa protheine ea lahleheloang mosebetsi lona tsa likokoana-hloko. Molao-motheo ena e ipapisitse le tšebeliso ea acid e carbolic (phenol ea metsi tharollo) e le ntho sebolaya dikokwana-hloko.

The tsitse ea sebopeho tlhaloganya sebaka, kgonego tse phahameng tsa ho hloleha tlas'a tšusumetso ea mahlahana a tse fapa-fapaneng ke haholo thata ho itšehla le ho etsa lipatlisiso protheine. Hape o bōpile bang mathata ha u sebelisa metsoako ka meriana le indasteri.

Ha denaturation protheine ile etsoang ke ho pepesehela thempereichara e phahameng, ea liehang tsidifatso etsahala maemo a itseng tshebetsong renativatsii - hlaphoheloa ba letsoalloa la (pele) conformation. Ha e le hantle sena ke bopaki ba hore ho raloe tsa ketane peptide ke ho latela sebōpeho sa mathomo.

Sebopeho sa letsoalloa conformation (mohloding sebakeng) ke tshebetso ho itlelang feela. Ka mantsoe a mang, tokisetso ena e tšoana le chelete e bonyane ba lokolohile eneji, le leng ka molek'hule e. E mong a ka ka lebaka leo ho fihlela qeto ea hore mohaho tlhaloganya sebaka sa ho kompone e khouthollotsweng ka tatelano amino acid e ka liketane le peptide. Sena se ka lehlakoreng le bolela hore polypeptides tsohle tse amanang ka ho alternation ea amino acid (ka mohlala, mioglobinovye peptide liketane), le tla nka e conformation tšoanang.

Liprotheine e ka ba ho se tšoane bohlokoa ka sebopeho sa mathomo, esita le haeba ho honyenyane kapa ho tšoanang ka ho feletseng ka conformation.